Figure 11

In vivo examination of mitochondrial motility and membrane potential following pre treatment with the mitochondrial PTP inhibitor CsA. Still images selected from time-lapse videos at time 0 and time 30 seconds following 10 μM CsA treatment and subsequent CMXRos staining. Mitochondria in time 30 seconds images have been false colored green to allow for comparative overlay images to demonstrate mitochondrial motility. Note that when the red and green overlap, the mitochondria appear yellow and are presumably still. A, D, G) time 0 seconds CMXRos stained images of CsA treated leaves corresponding with NPCD, EPCD and LPCD cells, respectively. B, E, H, K) time 30 seconds CMXRos stained images of CsA treated leaves corresponding with NPCD, EPCD and LPCD cells, respectively. C, F, I) Overlay of time 0 and 30 second still images corresponding with NPCD, EPCD and LPCD cells, respectively. These overlay images characterize the rapid mitochondrial movement in CsA treated leaves. Still images A, B and C taken from additional file 9. Still images D, E and F taken from additional file 10. Still images G, H and I taken from additional file 11. Scale bars (A-I) = 10 μm.