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Table 1 Summary of parameters, formulae and their description using data extracted from chlorophyll a fluorescence (OJIP) transient.

From: Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leaves

Fluorescence parameters

Description

F t

Fluorescence intensity at time t after onset of actinic illumination

F 50 μs

Minimum reliable recorded fluorescence at 50 μs with the PEA fluorimeter

Fk (F300 μs)

Fluorescence intensity at 300 μs

F P

Maximum recorded (= maximum possible) fluorescence at P-step

Area

Total complementary area between fluorescence induction curve and F = Fm

ABS

Absorption of energy

TR

Trap of energy

CS

Excited Cross section

Derived parameters (Selected OJIP parameters)

 

Fo≅F50 μs

Minimum fluorescence, when all PSII RCs are open

Fm = FP

Maximum fluorescence, when all PSII RCs are closed

Vj = (F2 ms -- Fo)/(Fm -- Fo)

Relative variable fluorescence at the J-step (2 ms)

Vi = (F30 ms -- Fo)/(Fm -- Fo)

Relative variable fluorescence at the I-step (30 ms)

WK= (F300 μs-- Fo/(Fj-- Fo)

Represent the damage to oxygen evolving complex OEC

Mo = 4 (F300 μs -- Fo)/(Fm-- Fo)

Approximated initial slope of the fluorescence transient

Yields or flux ratios

 

Ï•Po = TRo/ABS = 1-- (Fo/Fm) = Fv/Fm

Maximum quantum yield of primary photochemistry at t = 0

ϕEo = ETo/ABS = (Fv/Fm) × (1 -- Vj)

Quantum yield for electron transport at t = 0

ψEo = ETo/TRo = 1 -- Vj

Probability (at time 0) that a trapped exciton moves an electron into the electron transport chain beyond QA-

δRo = (1 -- Vi)/(1 -- Vj)

Efficiency with which an electron can move from the reduced intersystem, electron acceptors to the PSI end electron acceptors

Density of reaction centers.

RCQA = ϕPo × (ABS/CSm) × (Vj/Mo)

Amount of active PSII RCs (QA-reducing PSII reaction centers) per CS at t = m