Figure 2

Expression patterns of the FAF genes throughout development assayed by RNA in situ hybridization. (A-J) Expression of the FAF genes at the vegetative apex. Longitudinal (A-E) and transverse sections (F-J) through the vegetative apex hybridized with sense (A, F) and antisense probes (B-E, G-J) against the four FAF genes are shown. Highest expression was detected for FAF3 and FAF4 in the vascular and provascular tissue (D, E, I, J, arrows). (K-O) In inflorescences, FAF1 expression (L) was detected in the developing vasculature and young flowers. FAF2 expression (M) was highest in the inflorescence stem, but also detectable in the centre of the meristem (M, arrowhead). Expression of FAF3 was restricted to the developing vasculature (N), while FAF4 was also found in the centre of the meristem (O, arrowhead). No signal was found when sense probes were used (K). (P-V) During embryogenesis, FAF1 (Q), FAF3 (S), and FAF4 (T) were expressed in the embryo from heart stage onward, while expression of FAF2 was limited to the funiculus (V). Sense probes (P, U) did not result in any staining. Scale bars: 100 μm (A-O), 50 μm (P-T).