Figure 6

Recognition of the WRKY60 promoter by WRKY18 and WRKY40. A. Diagram of the WRKY60 gene, including the 1 kb upstream promoter that contains a cluster of three W-box sequences between -791 and -773 relative to the translation start codon. B. Nucleotide sequences of probes used for EMSA. PW60 contains three TTGAC sequences, which are mutated into TTGAA in mPW60. C. EMSA of binding of PW60 and mPW60 by recombinant WRKY18 protein (labelled as W18), WRKY40 protein (labelled as W40), and their mixture (labelled as W18+40). For each binding assay, 200 fmol recombinant proteins and 20 fmol labeled DNA probe were used.