Figure 8From: Structure and expression of the maize (Zea maysL.) SUN-domain protein gene family: evidence for the existence of two divergent classes of SUN proteins in plantsWestern blot of proteins ZmSUN3 and ZmSUN4. (A) Two peptide antibodies were made against synthetic peptides within (zms3gsp1a) and just after (zms3gsp2) the SUN-domain of the maize ZmSUN3 protein. The corresponding regions in ZmSUN3 and ZmSUN4 are aligned, and asterisks indicate divergent residues in ZmSUN4. (B) Western-blot detection (top panel) of ZmSUN3 and ZmSUN4 in various plant tissues. Protein was loaded on an equal-fresh-weight basis for leaf, root, silk, husk, earshoot, embryo, meiosis-stage tassel, and postmeiotic tassel, resulting in the detection of a single band of ~72 kDa. (C) Immunoblot showing the effect of increased sample boiling time on bands detected. Protein from meiosis-stage anthers appeared as a single band at ~70 kDa (arrow) after the protein was boiled in SDS for 10 min or more.Back to article page