Figure 3
From: Proteomic characterization of iron deficiency responses in Cucumis sativusL. roots
Clustering analysis. Two-way hierarchical clustering analysis of the 57 spots that showed at least a two-fold change in the relative spot volumes (Two-ways ANOVA, p > 0.001) with Fe and days of treatment as factors. The clustering analysis was performed with PermutMatrix graphical interface after Z-score normalization of the averages of relative spot values (n = 6). Pearson's distance and Ward's algorithm were used for the analysis. Each coloured cell represents the average of the relative spot value, according to the colour scale at the bottom of the figure. Spots labelled with asterisks are those subsequently identified by MS/MS.