Figure 4

Multiple sequence alignment of O. sativa CYPs. Human CyPA (hCyPA, GeneBank accession no. P62937) was used for comparison. The amino acids necessary for CsA binding as determined for hCYPA were marked by asterisks (*). Secondary structure features (a-helix and b-sheets) derived from the hCYPA were displayed. The targeting signal of putative chloroplast (thylakoid lumen) localized OsCYPs was manually adjusted (boxed): double Arg residues (--); the hydrophobic stretches following the double Args (---). Amino acids of full length protein for single CYP domain OsCYPs, and only the most conserved amino acids area for multiple CYP domain OsCYPs, were aligned. Backgrounds indicate percentage of amino acid similarity: black, 95%; dark grey, 70%; light grey, 40%.