Figure 6

Numbers of reads in the non-normalized and normalized 454 sequence analysis for genes encoding enzymes in the fatty acid and TAG biosynthetic pathways in bitter melon seeds. Numbers after the gene (a/b) are the numbers of reads for the corresponding genes in the 454 analysis of cDNA libraries: a, non-normalized; b, normalized. The dashed arrows in the plastid fatty acid biosynthetic pathway indicate one or more cycles of acyl-chain elongation that is initiated by 3-ketoacyl-ACP synthase (KAS) I or II. Abbreviations: BCCP, biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase; BC, biotin carboxylase subunit of acetyl-CoA carboxylase; α-CT, α-carboxyltransferase subunit of acetyl-CoA carboxylase; ACP, acyl carrier protein; FAB2/SAD, stearoyl-ACP desaturase; FatA, acyl-ACP thioesterase A; FatB, acyl-ACP thioesterase B; GPAT, glycerol 3-phosphate acyltransferase; LPAT, lysophosphatidic acid acyltransferase; PA Pase: phosphatidic acid phosphatase; LPCAT, lysophosphatidylcholine acyltransferase; AAPT, CDP-choline:diacyglyglycerol cholinephosphotransferase; PLC, phospholipase C-type enzymes; DGAT, diacylglycerol acyltransferase; PDAT, phospholipid:diacylglycerol acyltransferase; FAD2, Δ12 oleic acid desaturase; FAD3, Δ15 (ω-3) linoleic acid desaturase; G3P, glycerol-3-phosphate; LPA, lysophosphatidic acid; PA, phosphatidic acid; LPC, lysophosphatidylcholine; PC, phosphatidylcholine; DAG, diacylglycerol; TAG, triacylglycerol.