Table 2 Sequences of primers used for semi-quantitative RT-PCR
From: Characterisation of the Vitis viniferaPR10 multigene family
Gene | GenBank | Forward primer 5'→3' | Reverse primer 5'→ 3' |
|---|---|---|---|
Actin | AF369525 | TGCTATCCTTCGTCTTGACCTTG | GGACTTCTGGACAACGGAATCTC |
VvPR10.1 | AJ291705 | GAAATCATACAAGGAGAGGGAGGC | GCCAAACTTATTGAGACTGATAGGTG |
VvPR10.2 | AJ291704 | CGATCACAGTGTAGCGGAATGAGAAT | AAGCTATCAAGTGCGTGGAAGTCATT |
VvPR10.3 | EU379313 | GAAATCCTACAAGGACAGGGAGGT | CGGCCTTGGTGTGGTACTTTT |
VvPR10.4 | - | ATCCTTCCCCAAGCTATCAAG | GATTTGCCAAGAGGTAAGCC |
VvPR10.5 | - | ATCCTTGACTCTGATAACCTCA | ATGATATGAGACAAAGGAGTTTC |
VvPR10.6 | - | GTCCTTGATGTTGATAACCTC | AAGCCAAGCCTTTTAACTG |
VvPR10.7 | - | ATCGTCCCTCAGGCCATTA | AAGTGATTAAGTGGAGGAGAAGC |
VvPR10.8 | - | CTCTTGCCCCAGACCATAAG | ACATTGGACAACAGAGAAGTGAC |
VvPR10.9 | - | CAGTCAAAAGTACGCGACTCA | AAGTATAGGCGCGAGGGTGT |
VvPR10.10 | - | AATCAGTAAAGAGCATCGAGTT | AAAGTAATCACAACTCCTCGTC |