Development of Efficient Plant Regeneration and Transformation System for Impatiens Using Agrobacterium tumefaciensand Multiple Bud Cultures as Explants

Table 2 Mean percent of explants of I. walleriana cvs. Accent Red and Salmon Picote producing multiple shoots from HSCCN 4 weeks after placement on media with three growth regulator combinations.

Cultivar	TDZ/BA concentration	No. of explants	% of explants producing massive shoots/buds^x (mean ± standard error)
Accent Red	1 μM TDZ + 0 μM BA (IM1)	121	20.5 ± 6.4 A
	2.3 μM TDZ + 1.8 μM BA (IM2)	121	66.5 ± 6.4 B
	1 μM TDZ + 2.2 μM BA (IM3)	119	42.6 ± 6.4 C
Salmon	1 μM TDZ + 0 μM BA (IM1)	65	59.5 ± 5.3 A
Picote	2.3 μM TDZ + 1.8 μM BA (IM2)	68	80.1 ± 4.6 B
	1 μM TDZ + 2.2 μM BA (IM3)	65	43.9 ± 5.3 A

^x Treatments followed by different letters are significantly (P < 0.05) different using the LSD All-Pairwise Comparison Test (Statistix 9, Analytical Software, Tallahassee, FL, USA).

ISSN: 1471-2229