Substrate | Km(μM) | Vmax(pkat/mg) | Kcat/Km(nM-1. s-1) |
---|
Wild type | Â | Â | Â |
Tricetin | 59.51 ± 0.79 | 110 ± 7.0 | 74 |
5HFA | 270.98 ± 11.51 | 210 ± 6.0 | 31 |
V309I
| Â | Â | Â |
Tricetin | 101.08 ± 9.68 | 35 ± 8.0 | 13 |
5HFA | 18.24 ± 1.72 | 59 ± 2.0 | 130 |
- aThe affinity purified recombinant proteins (1.8 μg) were incubated with 5.0 to 80 μM of the indicated substrates, 1 mM of AdoMet containing 125 nCi of the [3H] label for 15 min at 30°C, and the activity in the products was determined as described in the Methods section. The data are averages of three determinations ± SE; pkat, the catalytic activity that raises the reaction rate by one pmol.s-1.